Isolation, Screening and Identification of Hydrocarbon Degrading Potential of Indigenous Fungus From Oil Contaminated Soil of Modha Para Automobile Shop of Raipur, C.G

The aim of this present investigation is to isolate, screen and identify hydrocarbon degrading fungi from oil contaminated soil of Modha Para automobiles of Raipur, C.G. During this study, nine pure fungal isolates were obtained through serial dilution of soil in potato dextrose agar. Primary screening tests were done to evaluate the growth diameter of fungal colonies on minimal salt media supplemented with 1% used engine oil (v/v). The fungal isolates MT 19, MT 18, MT 4 showed highest average growth rate. Later, confirmatory test was also performed for detecting the degrading capacity of isolated fungal strains through shake flask method by taking optical density at 600 nm by spectrophotometer. MT 19, MT 18, MT 4 gave higher percentage of oil degradation as compared to other isolates within 7 days. Morphological identification was performed and it was determined that fungal isolate MT 19 Aspergillus flavus gave 0.12 OD, MT 18 Aspergfillus fumigatus gave 0.15 OD and MT 4 Aspergillus nidulans gave 0.18 OD . It was found that MT 19 Aspergillus flavus gave higher % of degradation i.e. 76.9 % as compared to MT 18 Aspergfillus fumigates and MT 4 Aspergillus nidulans which were recorded as 71.1 % and 65.3 %.